Tests and Services Manual: Enteric Bacteriology
| CAMPYLOBACTER ID |
Specimen: |
Isolate |
|
Method: |
Conventional biochemicals | |
|
Turn Around Time: |
48 – 96 hrs | |
|
Special Requirements: |
RT on Campy BA media | |
| ENTERIC PATHOGEN PROFILE (Salmonella, Shigella, Yersinia, Campylobacter, Aeromonas, Pleisomonas, Enterohaemorrhagic Escherichia coli), Bacillus cereus, Clostridium perfringens |
Specimen: |
Stool |
|
Method: |
Culture; if positive or suspicious, conventional biochemicals and serotyping performed | |
|
Turn Around Time: |
3 – 10 days | |
|
Special Requirements: |
If stool, send in transport media (Cairy Blair) at Room Temperature. For DOHMH only. | |
| ID OF MISCELLANEOUS ENTEROBACTERIACEAE (Isolates that the referring laboratory does not consider to be either Salmonella nor Shigella) |
Specimen: |
Isolate |
|
Method: |
Conventional biochemicals and serotyping | |
|
Turn Around Time: |
3 – 10 days | |
|
Special Requirements: |
Send at Room Temperature | |
| SALMONELLA ID SEROTYPING (DOH Referrals Only) |
Specimen: |
Isolate |
|
Method: |
Conventional biochemicals (ID). PCR and serology (serotyping) | |
|
Turn Around Time: |
3 – 10 days | |
|
Special Requirements: |
Send at Room Temperature. DOHMH only | |
| SHIGELLA SPP., ID, SEROTYPING, and AST |
Specimen: |
Isolate |
|
Method: |
Conventional biochemicals serotyping, and AST | |
|
Turn Around Time: |
3 – 10 days | |
|
Special Requirements: |
Send at Room Temperature | |
| STX E. coli R/O |
Specimen: |
Stool or broth |
|
Method: |
Culture; Assay for Shiga toxin I & II performed if positive, ID and Serotyping | |
|
Turn Around Time: |
48 – 96 hrs | |
|
Special Requirements: |
Send stool and GN broth in cold pack | |
| STX E. coli Confirmation |
Specimen: |
Reflexive test, performed when E. coli/other EHEC is identified. |
|
Method: |
EIA and serotyping | |
|
Turn Around Time: |
48 – 96 hrs | |
|
Special Requirements: |
Isolate; send at Room Temperature | |
| VIBRIO CHOLERAE/ Vibrio spp. |
Specimen: |
Isolate |
|
Method: |
Conventional biochemicals and serotyping | |
|
Turn Around Time: |
3 – 10 days | |
|
Special Requirements: |
Send at Room Temperature | |
| Yersinia spp. |
Specimen: |
Isolate |
|
Method: |
Conventional biochemical | |
|
Turn Around Time: |
3 – 10 days | |
|
Special Requirements: |
Isolate; send at Room temperature |